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KMID : 0380620170490030258
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Korean Journal of Food Science and Technology
2017 Volume.49 No. 3 p.258 ~ p.264
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Comparison and validation of rutin and quercetin contents according to the extraction method of tartary Buckwheat (Fagopyrum tataricum Gaertn.)
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Kim Su-Jeong
Sohn Hwang-Bae
Kim Geum-Hee
Lee Yu-Young
Hong Su-Young
Kim Ki-Deog
Nam Jung-Hwan
Chang Dong-Chil
Suh Jong-Taek
Koo Bon-Cheol
Kim Yul-Ho
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Abstract
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The stability and accuracy of ultra-performance liquid chromatography (UPLC) used for evaluating the contents of rutin and quercetin in tartary buckwheat (Fagopyrum tataricum Gaertn.) seeds extracted by seven different extraction methods were determined. The seven extraction methods were reflux extraction (RE), ultra-sonification extraction (UE), stirrer extraction (SE), RE after UE (UE+RE), RE after SE (SE+RE), UE after SE (SE+UE), and RE with UE after SE (SE+UE+RE). Among the seven extraction methods used, RE yielded comparatively higher contents of rutin (2,277 mg/100 g) and quercetin (158 mg/100 g) than those yielded by other six extraction methods. The intra-day repeatability and inter-day precision of RE was 0.4-3.2% considering relative standard deviation (RSD), while accuracy was 88.8-102.4%. Therefore, RE with UPLC would be a rapid, accurate, and stable method for analyzing rutin and quercetin contents in tartary buckwheat.
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KEYWORD
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reflux extraction, rutin, quercetin, tartary buckwheat
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